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. 1998 Feb 20;279(5354):1190-3.
doi: 10.1126/science.279.5354.1190.

Probing single secretory vesicles with capillary electrophoresis

D T Chiu  1 , S J Lillard, R H Scheller, R N Zare, S E Rodriguez-Cruz, E R Williams, O Orwar, M Sandberg, J A Lundqvist
Affiliations

Probing single secretory vesicles with capillary electrophoresis

D T Chiu et al. Science. .

Abstract

Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individually with a combination of optical trapping, capillary electrophoresis separation, and a laser-induced fluorescence detection. With the use of optical trapping, a single vesicle that had attoliters (10(-18) liters) of volume was introduced into the tapered inlet of a separation capillary. Once the vesicle was injected, it was lysed, and its components were fluorescently labeled with naphthalene-2, 3-dicarboxaldehyde before separation. The resultant electropherograms indicated distinct variations in the contents of single vesicles.

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