Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Mar 1;26(5):1352-3.
doi: 10.1093/nar/26.5.1352.

Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes with CAG/CTG repeat expansions

M A Pujana  1 , V Volpini, X Estivill
Affiliations

Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes with CAG/CTG repeat expansions

M A Pujana et al. Nucleic Acids Res. .

Abstract

We report here a simple method for generating large CAG/CTG repeat sequences. We have applied this method to clone the genomic sequence containing the CAG/CTG repeat and its upstream intronic sequence present in spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) by a modified DIRECT method. With these modifications we have considerably simplified the generation of the repeat probe used to screen for anomalous bands. This method will facilitate the molecular approach to other genetic disorders where expansions of repeat sequences could be involved.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Cell. 1991 May 31;65(5):905-14 - PubMed
    1. Nat Genet. 1997 Sep;17(1):65-70 - PubMed
    1. Nature. 1991 Jul 4;352(6330):77-9 - PubMed
    1. Am J Hum Genet. 1992 Jul;51(1):7-9 - PubMed
    1. Nat Genet. 1994 Jan;6(1):14-8 - PubMed

Publication types

Substances