Engineering an enzyme to resist boiling
- PMID: 9482837
- PMCID: PMC19247
- DOI: 10.1073/pnas.95.5.2056
Engineering an enzyme to resist boiling
Abstract
In recent years, many efforts have been made to isolate enzymes from extremophilic organisms in the hope to unravel the structural basis for hyperstability and to obtain hyperstable biocatalysts. Here we show how a moderately stable enzyme (a thermolysin-like protease from Bacillus stearothermophilus, TLP-ste) can be made hyperstable by a limited number of mutations. The mutational strategy included replacing residues in TLP-ste by residues found at equivalent positions in naturally occurring, more thermostable variants, as well as rationally designed mutations. Thus, an extremely stable 8-fold mutant enzyme was obtained that was able to function at 100 degrees C and in the presence of denaturing agents. This 8-fold mutant contained a relatively large number of mutations whose stabilizing effect is generally considered to result from a reduction of the entropy of the unfolded state ("rigidifying" mutations such as Gly --> Ala, Ala --> Pro, and the introduction of a disulfide bridge). Remarkably, whereas hyperstable enzymes isolated from natural sources often have reduced activity at low temperatures, the 8-fold mutant displayed wild-type-like activity at 37 degrees C.
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Comment in
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Enzyme engineering reaches the boiling point.Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2035-6. doi: 10.1073/pnas.95.5.2035. Proc Natl Acad Sci U S A. 1998. PMID: 9482832 Free PMC article. Review. No abstract available.
References
-
- Agard D A. Science. 1993;260:1903–1904. - PubMed
-
- Lomas D A, Evans D L, Finch J T, Carrell R W. Nature (London) 1992;357:605–607. - PubMed
-
- Pepys M B, Hawkins P N, Booth D R, Vigushin D M, Tennent G A, Soutar A K, Totty N, Nguyen O, Blake C C F. Nature (London) 1993;362:553–557. - PubMed
-
- Chrunyk B A, Wetzel R. Protein Eng. 1993;6:733–738. - PubMed
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