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. 1998 Feb;41(2):215-23.
doi: 10.1002/1529-0131(199802)41:2<215::AID-ART5>3.0.CO;2-X.

Apoptotic cell clearance in systemic lupus erythematosus. II. Role of beta2-glycoprotein I

A A Manfredi  1 , P Rovere, S Heltai, G Galati, G Nebbia, A Tincani, G Balestrieri, M G Sabbadini
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Apoptotic cell clearance in systemic lupus erythematosus. II. Role of beta2-glycoprotein I

A A Manfredi et al. Arthritis Rheum. 1998 Feb.

Abstract

Objective: To analyze the contribution of beta2-glycoprotein I (beta2-GPI) to apoptotic cell recognition by antiphospholipid antibodies (aPL) and macrophages from patients with autoimmune disease.

Methods: Phosphatidylserine expression by Jurkat cells undergoing apoptosis upon CD95 crosslinking or ultraviolet irradiation was verified by confocal microscopy of cells stained with fluorescein isothiocyanate-labeled annexin V. Beta2-GPI was purified by heparin/cationic-exchange chromatography and was biotinylated or used to purify beta2-GPI-specific antibodies by affinity chromatography. Binding to apoptotic cells was assessed by flow cytometry. The clearance of 3H-labeled, apoptotic cells by macrophages was assessed by beta counting.

Results: The array of epitopes generated by beta2-GPI association with apoptotic cells specifically targets their recognition and is required for their opsonization by human aPL. Nevertheless, beta2-GPI is not required for apoptotic cell clearance by human macrophages in the absence of aPL.

Conclusion: The proinflammatory clearance of aPL-opsonized apoptotic cells, but not the nonphlogistic clearance of apoptotic cells by scavenger macrophages, depends on beta2-GPI.

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