Transmission electron and atomic force microscopic observation of polysomes on carbon-coated grids prepared by surface spreading

Abstract

A simple modification of surface spreading that enables easy examination of subcellular structures by both transmission electron microscopy (TEM) and atomic force microscopy (AFM) was developed in the present study. Specimens were adsorbed onto carbon-coated electron microscopy (EM) grids with parlodion backing for comparison of images obtained using TEM and AFM. Fine structures of ribosomes and polysomes were observed in this study. Washing the mounted specimen with detergent such as 0.05% Photo-Flo 200 before conducting negative staining or metal shadowing brought clear visualization of ribosomes and polysomes. The same preparation could apply to AFM imaging without any additional treatment.