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. 1976 Jun 15;156(3):561-8.
doi: 10.1042/bj1560561.

A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein

T J Humphrey, D D Davies

A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein

T J Humphrey et al. Biochem J. .

Abstract

A method for measuring the rate of protein degradation is described. The method measures the change in 2-3H content of protein with time by racemization of the protein hydrolysate with acetic anhydride. The 3H on C-2 of amino acids is stable in proteins but becomes labile, owing to the action of transaminases, once the amino acids are released by proteolysis. The specific measurement of 2-3H in amino acids largely overcomes problems due to compartmentation and isotope recycling and evidence to support this claim is presented. Values for the half-life of Lemna minor (duckweed) protein determined by the new method are compared with values obtained by other methods.

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