The hypothalamo-hypophysial system in acipenseridae. VI. The proximal neurosecretory contact region

Abstract

The proximal neurosecretory contact region (PCR) of Acipenseridae, a homologue of the tetrapod median eminence, has been studied by light, fluorescence and electron microscopy. It occupies the rostral and chiefly the ventral surfaces of the hypothalamic tuber cinereum. PAF-positive fibres occur in the zone of the preoptico-hypophysial tract but their terminal enlargements are concentrated mainly in the external zone. They make contact with the primary portal capillaries situated in the pia mater. Monoaminergic fibres and terminals with an intense green fluorescence are localized in the same regions. The fibres of some bipolar monoaminergic neurons of the PCR make contact both with the third ventricle and the primary portal capillaries. Three types of granule-containing neurosecretory fibres and terminals have been recognized in the PCR. Fibres of types A1 (d = 120-300 nm) and A2 (D = 100-170 nm) are peptidergic PAR-positive, although some fibres, including some of type A1, belong possibly to PAF-negative type. Monoaminergic type B fibres have granules 80-100 nm in diameter. Neurosecretory terminals and vascular "endfeet" of tanycytes make contact with the 70 nm thick outer basement membrane of the primary portal capillaries. Several laminae of thin horizontally oriented tanycyte processes form a boundary between the external zone and the preoptico-hypophysial tract. Few neuroglial cells with pale cytoplasm, numerous lysosomes and lipofuscin granules are seen in this region. It is hypothesized that, as in other vertebrates both peptide hypophysiotropic neurohormones and monoamines are discarged from the PCR into the portal circulation and affect the activity of the glandular cells of the pars distalis.