Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Oct;50(5):276-8.
doi: 10.1136/mp.50.5.276.

High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining

T Xie  1 , S L Ho, O C Ma
Affiliations

High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining

T Xie et al. Mol Pathol. 1997 Oct.

Abstract

Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1991 May 11;19(9):2500 - PubMed
    1. Trends Genet. 1992 Feb;8(2):49 - PubMed
    1. Nucleic Acids Res. 1991 Jan 25;19(2):405-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766-70 - PubMed
    1. Nucleic Acids Res. 1993 Aug 11;21(16):3637-42 - PubMed