[Structure and chromosomal localization of human neurogranin gene]

Abstract

A 12.5-kb DNA fragment containing the entire human neurogranin gene (hng) was isolated from the genomic phage library using the human neurogranin (hNG) cDNA as a probe. The gene consists of four exons and three introns. The first two exons include the nucleotide sequence that encodes the complete 78-amino acid sequence of neurogranin. It is shown that the genome organization of hug is essentially similar to that of the homologous rat neurogranin gene (rng). The hng gene has several potential transcription start sites, and the promoter region is characterized by the absence of the CCAAT and TATA boxes in the proximal region upstream from the transcription start sites. The 5'-flanking region of hng has a domain with multiple AT-rich motifs, which is similar to the region present nearly at the same position in the promoter of rng. It was shown by fluorescence in situ hybridization that hng is located on chromosome 11 in the region 11q23.3-q24.1.