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. 1976 Jun 1;65(2):325-31.
doi: 10.1111/j.1432-1033.1976.tb10345.x.

Peroxidase from human cervical mucus. The isolation and characterisation

Free article

Peroxidase from human cervical mucus. The isolation and characterisation

J S Shindler et al. Eur J Biochem. .
Free article

Abstract

A peroxidase has been isolated from pooled specimens of human cervical mucus. Investigation of substrate and inhibitor specificity, pH optimum, chromatography on Sephadex G-200, disc electrophoresis, ultraviolet absorption spectrum and the effects of temperature and ionic strength variation are described. A study of the steady-state kinetics is reported using hydrogen peroxide and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as substrates and a catalytic mechanism is proposed.

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