Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Jan;62(1):16-21.
doi: 10.1271/bbb.62.16.

Casein phosphopeptides from casein micelles by successive digestion with pepsin and trypsin

T Ono  1 , Y Takagi, I Kunishi
Affiliations
Free article

Casein phosphopeptides from casein micelles by successive digestion with pepsin and trypsin

T Ono et al. Biosci Biotechnol Biochem. 1998 Jan.
Free article

Abstract

When milk is ingested, casein micelles will be successively digested by pepsin in the stomach and trypsin in the intestine. Therefore, we digested casein micelles successively with pepsin at pH 4.0 and trypsin at pH 7.0, and recovered casein phosphopeptides (CPP) as CPP-calcium phosphate (CP) complexes. The CPP-CP complexes contained 248 mg of calcium/g peptides and 175 mg of inorganic phosphorus/g peptides, which were higher than those of CPP-CP complexes driven from acid-precipitated casein and casein micelles by tryptic digestion only. It contained more alpha S1-CN-5P(f59-79) than the other CPP preparations did.

PubMed Disclaimer