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. 1976 Jun;94(2):395-9.
doi: 10.1099/00221287-94-2-395.

Effect of growth temperature on the cryopreservation of prototheca

G J Morris

Effect of growth temperature on the cryopreservation of prototheca

G J Morris. J Gen Microbiol. 1976 Jun.

Abstract

The temperature at which Prototheca spp. were grown determined their response to freezing to -196 degrees C and subsequent thawing. Cells cultured at 35 degrees C were the most sensitive to freezing injury; at lower growth temperatures, resistance to freezing damage was seen. At all culture temperatures examined, the freezing tolerance varied with the age of the culture.

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