Evaluation of a new enrichment broth for the isolation of Salmonella spp. from poultry products

Abstract

Four selective enrichment broths were compared for the detection of Salmonella spp. in naturally contaminated poultry products and the recovery of atypical Salmonella strains in suspensions of pure cultures. In analysis of 100 poultry samples, the sensitivities observed were 94.0% for Müller-Kauffmann Tetrathionate-Brilliant Green (MKTBG), 97.6% for Rappaport Vassiliadis (RV), 42.2% for Selenite Cystine (SC) and 97.6% for the new broth KIMAN (Whitley Impedance Broth supplemented with 20 mg/l of novobiocin sodium salt, 10 mg/l of malachite green oxalate and 40 g/l of potassium iodide). The two most efficient broths--RV and KIMAN for recovery of atypical Salmonella strains (gallinarum biotypes gallinarum and pullorum, typhi, paratyphi A) were less toxic than MKTBG but more toxic than s.c. broth. According to these results, the use of RV and KIMAN could be a good combination to assure maximal recovery of Salmonella strains.