Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Sep;1(3):255-70.
doi: 10.1007/BF02783667.

Cytotoxic and fluorescent assays for thymocyte subpopulations differing in surface thy-1 level

K Shortman, D S Linthicum, F L Battye, I Goldschneider, A Liabeuf, P Golstein, E A Clark, P Lake

Cytotoxic and fluorescent assays for thymocyte subpopulations differing in surface thy-1 level

K Shortman et al. Cell Biophys. 1979 Sep.

Abstract

A number of analytical techniques for distinguishing and separating the "high theta" "low theta" subpopulations of mouse thymocytes have been compared. A differential cytotoxic assay was compared to a quantitative immunofluorescent assay on individual cells using flow cytofluorometry and cell sorting. Conventional anti-Thy-1 antisera were compared with a monoclonal IgM anti-Thy-1. The monoclonal reagent greatly improved both types of assay, eliminated a number of artifacts and allowed either procedure to be used to give a clear distinction, based on Thy-1 level, between the two subpopulations. The distribution of Thy-1 on thymocytes is bimodal, rather than continuous. These separate "high theta" and "low theta" categories each includes a population a population of dividing cells.

PubMed Disclaimer

References

    1. Fed Proc. 1975 Feb;34(2):141-4 - PubMed
    1. Prog Allergy. 1972;16:214-99 - PubMed
    1. Nature. 1977 Apr 7;266(5602):550-2 - PubMed
    1. Nat New Biol. 1971 Feb 10;229(6):182-4 - PubMed
    1. Cell Immunol. 1975 Jan;15(1):109-28 - PubMed

LinkOut - more resources