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. 1998 Feb;42(2):444-6.
doi: 10.1128/AAC.42.2.444.

The Cys607-->Tyr change in the UL97 phosphotransferase confers ganciclovir resistance to two human cytomegalovirus strains recovered from two immunocompromised patients

F Baldanti  1 , M R Underwood, C L Talarico, L Simoncini, A Sarasini, K K Biron, G Gerna
Affiliations

The Cys607-->Tyr change in the UL97 phosphotransferase confers ganciclovir resistance to two human cytomegalovirus strains recovered from two immunocompromised patients

F Baldanti et al. Antimicrob Agents Chemother. 1998 Feb.

Abstract

Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains recovered from an AIDS patient (strain VR4990) and a heart transplant recipient (strain VR5474) showed a Cys607-->Tyr change in the UL97-encoded phosphotransferase. No amino acid substitutions were observed in the viral DNA polymerase. Marker transfer experiments showed marked reduction in GCV phosphorylation and drug susceptibility of the recombinant HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involved in GCV substrate recognition.

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Figures

FIG. 1
FIG. 1
Anabolism of GCV by HCMV strains. MRC-5 cells were mock infected or infected with UL97 recombinant HCMV strain VR4990rec2-1-1 or its parental strains: clinical isolate VR4990 (GCV resistant) and reference strain AD169 (GCV sensitive). For comparison, GCV anabolism in cells infected with reference GCV-resistant HCMV strain XbaF4-3-1, derived by transfer of UL97 from 759rD100 back into AD169 (22, 23), was evaluated.
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References

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