Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Apr;66(4):1783-6.
doi: 10.1128/IAI.66.4.1783-1786.1998.

Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells

Y Fu  1 , G Rieg, W A Fonzi, P H Belanger, J E Edwards Jr, S G Filler
Affiliations

Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells

Y Fu et al. Infect Immun. 1998 Apr.

Abstract

To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1. These results indicate that ALS1 may encode a candidal adhesin.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
S. cerevisiae transformed with pYF-5 exhibits increase adherence to endothelial and epithelial cells. S. cerevisiae cells were transformed with either pYF-5 or the empty plasmid (pYesR). They were grown in either glucose (Glu) to suppress expression of the candidal gene or galactose (Gal) to induce expression of the candidal gene. Next, the adherence of these organisms to human umbilical vein endothelial cells (A) or FaDu epithelial cells (B) was determined. Results are the medians and interquartile ranges from at least four separate experiments, each performed in triplicate.
FIG. 2
FIG. 2
Comparison of the published sequence of ALS1 (top) with the sequence of ALS1 contained in plasmid pYF-5 (bottom). GPI, glycosylphosphatidylinositol.
See this image and copyright information in PMC

References

    1. Elledge S J, Mulligan J T, Ramer S W, Spottswood M, Davis R W. Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc Natl Acad Sci USA. 1991;88:1731–1735. - PMC - PubMed
    1. Filler S G, Pfunder A S, Spellberg B J, Spellberg J P, Edwards J E., Jr Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect Immun. 1996;64:2609–2617. - PMC - PubMed
    1. Fu, Y., S. G. Filler, B. J. Spellberg, W. A. Fonzi, A. S. Ibrahim, T. Kanbe, M. A. Ghannoum, and J. E. Edwards, Jr. Cloning and characterization of CAD1/AAF1, a gene from Candida albicans that induces adherence to endothelium when expressed in Saccharomyces cerevisiae. Infect. Immun., in press. - PMC - PubMed
    1. Gaur N K, Klotz S A. Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins. Infect Immun. 1997;65:5289–5294. - PMC - PubMed
    1. Ghannoum M A, Filler S G, Ibrahim A S, Fu Y, Edwards J E., Jr Modulation of the interactions of Candida albicans and endothelial cells by fluconazole and amphotericin B. Antimicrob Agents Chemother. 1992;36:2239–2244. - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources