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. 1998 Jan:144 ( Pt 1):193-199.
doi: 10.1099/00221287-144-1-193.

Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

Pei Pei Chong  1 , Sylvia M Podmore  1 , Helen M Kieser  2 , Matthias Redenbach  3 , Kürsad Turgay  4 , Mohamed Marahiel  4 , David A Hopwood  2 , Colin P Smith  1
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Free article

Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

Pei Pei Chong et al. Microbiology (Reading). 1998 Jan.
Free article

Abstract

Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single approximately 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

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