Tissue-specific forms of actin in the developing chick

Abstract

Actin has been identified in nonmuscle and muscle tissues as a highly conserved homogeneous protein. We have identified and characterized actin from embryonic and adult chick brain and muscle, and have compared these actins by SDS and urea/SDS gradient polyacrylamide gel electrophoresis. In the presence of SDS alone, embryonic or adult brain and muscle actin co-migrate as homogeneous polypeptides. Electrophoresis of both actins in the presence of urea and SDS, however, reveals that brain and muscle actins migrate with distinctly different mobilities. Actin from embryonic thigh muscle at different stages of development migrates as two separate components. In early muscle development, only the "brain" type actin is present. As muscle development progresses the "muscle" type actin becomes relatively more abundant, so that by day 20 of embryonic development, "muscle" actin becomes predominant. These results may be interpreted as due to differences in the primary structure of actin.