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. 1998 Apr;36(4):971-4.
doi: 10.1128/JCM.36.4.971-974.1998.

Epidemiological analysis of Salmonella enteritidis isolates from humans and broiler chickens in Thailand by phage typing and pulsed-field gel electrophoresis

S Boonmar  1 , A Bangtrakulnonth, S Pornrunangwong, J Terajima, H Watanabe, K Kaneko, M Ogawa
Affiliations

Epidemiological analysis of Salmonella enteritidis isolates from humans and broiler chickens in Thailand by phage typing and pulsed-field gel electrophoresis

S Boonmar et al. J Clin Microbiol. 1998 Apr.

Abstract

To determine the phage types (PT) of Salmonella enteritidis found in Thailand and to clarify the potential for human infection by S. enteritidis in broiler chicken meat, human and poultry isolates taken from Thailand between 1990 and 1997 were phage typed and analyzed by pulsed-field gel electrophoresis (PFGE). Ten different PT were found among the 302 isolates phage typed, with PT 4 being the most frequent in human (73.9%) and poultry (76.2%) isolates, followed by PT 1 (8.0%), 8 (3.6%), and 7a (2.2%) in human isolates and by PT 7a (4.9%), 1 (3.7%), and 12 (2.4%) in poultry isolates. Of the 53 isolates analyzed by PFGE, 45 showed an indistinguishable pattern (pattern A) by BlnI-digested PFGE and the other 8 isolates showed a very similar pattern that differed by only a few bands. These results indicate the spread of a genetically identical clone of S. enteritidis in humans and poultry in Thailand.

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Figures

FIG. 1
FIG. 1
PFGE patterns of S. enteritidis isolates collected from Thai humans and poultry from 1990 to 1997 and digested with BlnI. Lanes: L, lambda ladder used as molecular size markers; A, pattern A derived from 45 isolates of S. enteritidis from humans and poultry; B to E, patterns B to E from four human isolates collected in 1990; F and G, patterns F and G from isolates from chicken feces collected in 1993 and 1994; H and I, patterns H and I from isolates from retail chicken meat collected in 1996 and 1997.
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