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. 1998 Apr;36(4):1117-21.
doi: 10.1128/JCM.36.4.1117-1121.1998.

Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid

P Crepin  1 , L Audry, Y Rotivel, A Gacoin, C Caroff, H Bourhy
Affiliations

Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid

P Crepin et al. J Clin Microbiol. 1998 Apr.

Abstract

An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.

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