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. 1998 Apr;36(4):1125-7.
doi: 10.1128/JCM.36.4.1125-1127.1998.

Development of a monoclonal antibody to a Ureaplasma urealyticum serotype 9 antigen

A Naessens  1 , X Cheng, S Lauwers, J A Robertson
Affiliations

Development of a monoclonal antibody to a Ureaplasma urealyticum serotype 9 antigen

A Naessens et al. J Clin Microbiol. 1998 Apr.

Abstract

We produced a monoclonal antibody (MAb) to Ureaplasma urealyticum Vancouver, the serotype 9 standard strain. By immunoblotting, this MAb showed a single, 85-kDa band with the homologous serotype and a minor, 100-kDa band with serotype 2 but did not react with any other serotype standard strain. Clinical isolates of U. urealyticum were tested with this MAb and with two sets of polyclonal antisera against the 14 serotype standard strains. The use of MAb 9-2H9 correctly identified certain serotype 9 strains but did not react with wild-type strains lacking the serotype 9 determinant.

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Figures

FIG. 1
FIG. 1
Immunoblot showing the reaction pattern of MAb 9-2H9 (immunoglobulin G2a, specific to serotypes 2 and 9). Lanes 1 to 14 contain whole-cell preparations of the 14 serotype standard strains of U. urealyticum. Molecular masses of the bands, in kilodaltons, are indicated to the left of the gel.
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