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. 1998 Apr;36(4):1151-3.
doi: 10.1128/JCM.36.4.1151-1153.1998.

Diagnosis of Mycoplasma pneumoniae infection in autopsy and open-lung biopsy tissues by nested PCR

D F Talkington  1 , W L Thacker, D W Keller, J S Jensen
Affiliations

Diagnosis of Mycoplasma pneumoniae infection in autopsy and open-lung biopsy tissues by nested PCR

D F Talkington et al. J Clin Microbiol. 1998 Apr.

Abstract

A nested PCR specific for the Mycoplasma pneumoniae P1 gene was used to diagnose mycoplasma infection in two cohort patients with severe pneumonia within 24 h of tissue receipt. A postmortem diagnosis of M. pneumoniae infection was obtained for the first patient, who died without the collection of appropriate paired samples for serodiagnosis. An open-lung biopsy obtained from the second patient allowed a quick, definitive diagnosis and proper selection of therapy.

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Figures

FIG. 1
FIG. 1
Nested M. pneumoniae PCR of lung tissue. Lanes: A, 100-bp molecular size markers; B, open-lung biopsy tissue from patient 2; C, autopsy lung tissue from patient 1; D, negative control; E, positive control showing 107-bp amplicon.
See this image and copyright information in PMC

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