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. 1976 May-Jun;25(3):245-50.

Valyl-transfer RNA formation stimulated by monovalent cations and spermine in rat liver

M Romano, M L Lombardi
  • PMID: 955857

Valyl-transfer RNA formation stimulated by monovalent cations and spermine in rat liver

M Romano et al. Ital J Biochem. 1976 May-Jun.

Abstract

Valyl-tRNA synthetase from rat liver catalyzes the aminoacylation of tRNA in the presence of a) Mg2+, b) monovalent cations and c) spermine. The degree of aminoacylation with Na+ is equal to that with Mg2+ but the ATP required with Na+ is 25 times lower than that with Mg2+. The aminoacylation becomes almost undetectable in the presence of Na+ when tRNA deprived of Mg2+ is used.

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