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. 1998 Apr;42(4):818-20.
doi: 10.1128/AAC.42.4.818.

In vitro evaluation of drug susceptibilities of Babesia divergens isolates

P Brasseur  1 , S Lecoublet, N Kapel, L Favennec, J J Ballet
Affiliations

In vitro evaluation of drug susceptibilities of Babesia divergens isolates

P Brasseur et al. Antimicrob Agents Chemother. 1998 Apr.

Abstract

The susceptibilities of three bovine and two human Babesia divergens isolates to antimicrobial agents were evaluated in vitro by a tritiated hypoxanthine incorporation assay. The MICs at which 50% of isolates are inhibited (MIC50s) for mefloquine (chlorhydrate), chloroquine (sulfate), quinine (chlorhydrate), clindamycin (phosphate), pentamidine (isethionate), phenamidine (isethionate) plus oxomemazine (chlorhydrate), lincomycin (chlorhydrate monohydrate), and imidocarb (dipropionate) were determined. Except for imidocarb, the MIC50s observed for the different isolates were close. Imidocarb and the combination of phenamidine plus oxomemazine exhibited the highest in vitro activity, while antimalarial agents such as mefloquine, choroquine, and quinine were inactive. Other drugs had intermediate activities. The data support further in vitro evaluation of antimicrobial agents active against B. divergens for the improvement of therapeutic strategies.

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Figures

FIG. 1
FIG. 1
Comparison of MIC50s of clindamycin and imidocarb for the Weybridge 8843 bovine isolate and the Rouen human isolate obtained in the [3H]hypoxanthine incorporation assay and by microscopic examination. Results are expressed as mean values for 15 independent experiments (one bar indicates 1 SD).
FIG. 2
FIG. 2
MIC50s of phenamidine plus oxomemazine and of imidocarb for bovine B. divergens isolates. Results are expressed as mean values for eight independent experiments (one bar indicates 1 SD).
FIG. 3
FIG. 3
MIC50s of clindamycin and clindamycin plus quinine for bovine and human isolates of B. divergens in 18 independent experiments. Results are expressed as mean values for 18 independent experiments (one bar indicates 1 SD).
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