Simultaneous determination of creatine, uric acid, creatinine and hippuric acid in urine by high performance liquid chromatography

Abstract

A simple method for the simultaneous determination of creatine, uric acid, creatinine and hippuric acid in urine using reversed-phase high performance liquid chromatography was described. Chromatography was performed on a Nova-Pak C18 (3.9 x 150 mm) column with a mobile phase of 0.02 mol/L KH2PO4, and UV detection at 220 nm. The recoveries of creatine, uric acid, creatinine and hippuric acid were 103.13, 99.86, 98.26 and 98.24%, respectively. The calibration curves were linear within the concentration range of 2-200 micrograms/mL for creatine, uric acid and hippuric acid, and 2-400 micrograms/mL for creatinine. The detection limits of the method were 0.69, 0.21, 0.10 and 0.095 microgram/mL for creatine, uric acid, creatinine and hippuric acid, respectively. This method has been applied to the analysis of urine samples from normal subjects and some patients.