Activities of aromatase and 3beta-hydroxysteroid dehydrogenase/delta4-delta5 isomerase in whole organ cultures of tissues from developing zebra finches

Abstract

The hormonal basis for masculine song development in the zebra finch remains unidentified. To understand how steroids are differentially supplied to the brains of males and females to cause sexually dimorphic development of this behavior, we have studied the steroidogenic capability of zebra finch tissues during early development (1 to 8 days posthatching). Here, we report on the use of cultures of whole gonads, adrenals, and telencephalons to measure the activities of two steroidogenic enzymes: aromatase, the enzyme that catalyzes the conversion of androgen to estrogen, and 3beta-hydroxysteroid dehydrogenase/delta4-delta5 isomerase (3beta-HSD), the enzyme that converts pregnenolone into progesterone. We also examined the effect of cAMP on aromatase activity in these tissues as this intracellular second messenger has been shown previously to regulate aromatase in both central and peripheral tissues of other species. In untreated cultures, aromatase was detected at the highest levels in male and female telencephalon and in ovary. Dibutyryl (dB)-cAMP had no significant effect on aromatase activity in any tissue. However, after dB-cAMP treatment, estrogens were regularly detected in cultures of whole testes. Although this activity was relatively low when compared to total activity found in other tissues, due to the small size of the testes at this age of development, the specific activity (per milligram of protein) might be high enough to produce some estrogen. Adrenal aromatase was unconfirmed in the presence or absence of cAMP. 3Beta-HSD activity was undetected in brain but was detected in gonads and adrenals from all birds. There were no significant differences in gonadal or adrenal 3beta-HSD activity between males and females. Although these data present the first evidence for testicular aromatase in the zebra finch, they provide no evidence to support a mechanism to generate a greater estrogenic signal in male zebra finches after hatching.