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. 1998 May;64(5):1721-4.
doi: 10.1128/AEM.64.5.1721-1724.1998.

Improved isolation of Vibrio vulnificus from seawater and sediment with cellobiose-colistin agar

L Høi  1 , I Dalsgaard, A Dalsgaard
Affiliations

Improved isolation of Vibrio vulnificus from seawater and sediment with cellobiose-colistin agar

L Høi et al. Appl Environ Microbiol. 1998 May.

Abstract

An improved selective medium, cellobiose-colistin (CC) agar, gave a significantly higher (P < 0.05) isolation rate of Vibrio vulnificus from water and sediment samples than did modified cellobiose-polymyxin B-colistin (mCPC) agar. In a total of 446 alkaline peptone water preenrichments amended with polymyxin B, V. vulnificus was isolated from 154 preenrichments (35%) with mCPC agar and from 179 preenrichments (40%) with CC agar. CC agar gave a higher plating efficiency of V. vulnificus cells than did cellobiose-polymyxin B-colistin (CPC) agar, mCPC agar, or thiosulfate-citrate-bile salts-sucrose (TCBS) agar; the only significant difference was observed with TCBS agar, which gave much lower plating efficiencies than the other selective media. Determination of MICs demonstrated that the concentrations of colistin and polymyxin B in CPC agar inhibit growth of a proportion of V. vulnificus strains.

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Figures

FIG. 1
FIG. 1
Distribution of MICs of colistin for 50 V. vulnificus strains. Arrows indicate the concentrations of colistin in CPC, mCPC, and CC agars.
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References

    1. Barrow G I, Feltham R K A, editors. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge, United Kingdom: Cambridge University Press; 1993.
    1. Brayton P R, West P A, Russek E, Colwell R R. New selective plating medium for isolation of Vibrio vulnificus biogroup 1. J Clin Microbiol. 1983;17:1039–1044. - PMC - PubMed
    1. Bryant R G, Jarvis J, Janda J M. Use of sodium dodecyl sulfate-polymyxin B-sucrose medium for isolation of Vibrio vulnificus from shellfish. Appl Environ Microbiol. 1987;53:1556–1559. - PMC - PubMed
    1. Dalsgaard A, Dalsgaard I, Høi L, Larsen J L. Comparison of a commercial biochemical kit and an oligonucleotide probe for identification of environmental isolates of Vibrio vulnificus. Lett Appl Microbiol. 1996;22:184–188. - PubMed
    1. Dalsgaard A, Frimodt-Møller N, Bruun B, Høi L, Larsen J L. Clinical manifestations and epidemiology of Vibrio vulnificus infections in Denmark. Eur J Clin Microbiol Infect Dis. 1996;15:227–231. - PubMed

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