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. 1998 May;64(5):1721-4.
doi: 10.1128/AEM.64.5.1721-1724.1998.

Improved isolation of Vibrio vulnificus from seawater and sediment with cellobiose-colistin agar

L Høi  1 , I Dalsgaard, A Dalsgaard
Affiliations

Improved isolation of Vibrio vulnificus from seawater and sediment with cellobiose-colistin agar

L Høi et al. Appl Environ Microbiol. 1998 May.

Abstract

An improved selective medium, cellobiose-colistin (CC) agar, gave a significantly higher (P < 0.05) isolation rate of Vibrio vulnificus from water and sediment samples than did modified cellobiose-polymyxin B-colistin (mCPC) agar. In a total of 446 alkaline peptone water preenrichments amended with polymyxin B, V. vulnificus was isolated from 154 preenrichments (35%) with mCPC agar and from 179 preenrichments (40%) with CC agar. CC agar gave a higher plating efficiency of V. vulnificus cells than did cellobiose-polymyxin B-colistin (CPC) agar, mCPC agar, or thiosulfate-citrate-bile salts-sucrose (TCBS) agar; the only significant difference was observed with TCBS agar, which gave much lower plating efficiencies than the other selective media. Determination of MICs demonstrated that the concentrations of colistin and polymyxin B in CPC agar inhibit growth of a proportion of V. vulnificus strains.

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Figures

FIG. 1
FIG. 1
Distribution of MICs of colistin for 50 V. vulnificus strains. Arrows indicate the concentrations of colistin in CPC, mCPC, and CC agars.
See this image and copyright information in PMC

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