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. 1998 May;180(9):2556-9.
doi: 10.1128/JB.180.9.2556-2559.1998.

The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine

J L Schreve  1 , J K Sin, J M Garrett
Affiliations

The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine

J L Schreve et al. J Bacteriol. 1998 May.

Abstract

The yeast YCC5 gene encodes a putative amino acid permease and is homologous to GNP1 (encoding a high-affinity glutamine permease). Using strains with disruptions in the genes for multiple permeases, we demonstrated that Ycc5 (which we have renamed Agp1) is involved in the transport of asparagine and glutamine, performed a kinetic analysis of this activity, and showed that AGP1 expression is subject to nitrogen repression.

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Figures

FIG. 1
FIG. 1
Kinetics of asparagine uptake in JGY51 (gap1 gnp1) and JGY52 (gap1 gnp1 agp1). The rate of [14C]asparagine uptake was determined in JGY51 (filled symbols) and JGY52 (open symbols) as described previously (20, 26). The data was plotted as Michaelis-Menten (A) and Eadie-Hofstee (B) graphs. The Michaelis-Menten graphs were fitted to curves assuming two permeases (JGY51) and one permease (JGY52) with the Solver program of Microsoft Excel. Each data point is the average of at least three measurements, with a standard error of <10%. The deduced Kmapp for asparagine of Agp1 is 0.29 mM, and the Vmax is 9.0 nmol/min/mg (dry weight).
FIG. 2
FIG. 2
Kinetics of glutamine uptake in JGY51 (gap1 gnp1) and JGY52 (gap1 gnp1 agp1). The rate of [14C]glutamine uptake was determined as described in the legend for Fig. 1. The data were plotted as Michaelis-Menten (A) and Eadie-Hofstee (B) graphs. Each data point is the average of at least two measurements, with a standard error of <15%. The deduced Kmapp for glutamine of Agp1 is 0.79 mM, and the Vmax is 7.5 nmol/min/mg (dry weight).
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