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. 1976 Jul;47(2):367-70.
doi: 10.1530/jrf.0.0470367.

The influence of in-vitro culture and cooling on the survival and development of cow embryos

The influence of in-vitro culture and cooling on the survival and development of cow embryos

A O Trounson et al. J Reprod Fertil. 1976 Jul.

Abstract

Cow morulae cultured in a phosphate-buffered medium containing serum developed normally and retained viability when transferred to recipients. Unlike earlier cleavage stages, cow blastocysts tolerated cooling to 0 degrees C and retained viability after storage for 48 hr at 0 degrees C when transferred to recipients.

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