Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 May;36(5):1446-9.
doi: 10.1128/JCM.36.5.1446-1449.1998.

Survey of Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases: prevalence of SHV-12 and SHV-2a in Korea

J Kim  1 , Y Kwon, H Pai, J W Kim, D T Cho
Affiliations

Survey of Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases: prevalence of SHV-12 and SHV-2a in Korea

J Kim et al. J Clin Microbiol. 1998 May.

Abstract

Fifty-three clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae collected from three hospitals in Korea were investigated for phenotypical and genotypical characterizations. Among these, 39 strains (74%) were shown by isoelectric focusing to carry SHV-type beta-lactamases: 27 strains showed the pI 8.2 beta-lactamase, and another 12 strains showed the pI 7.6 beta-lactamase. The SHV gene of each of these strains was amplified by PCR, followed by nucleotide sequencing analysis. The gene of the pI 8.2 beta-lactamase was found to be identical to the sequences encoding SHV-12, and the gene of the pI 7.6 beta-lactamase was identical to the sequences encoding SHV-2a. A total of eight cefoxitin-resistant strains were found to have the plasmid-mediated AmpC-type beta-lactamase, with a pI of 8.0, and this was confirmed to be CMY-1 beta-lactamase by PCR and hybridization analysis. Noteworthy in this study is the fact that SHV-12 and SHV-2a have been the most commonly identified SHV-type ESBLs in Korea.

PubMed Disclaimer

References

    1. Ambler R P, Coulson A F W, Frere J M, Ghuysen J M, Joris B, Forsman M, Levesque R C, Tiraby G, Waley S G. A standard numbering scheme for the class A β-lactamases. Biochem J. 1991;276:269–270. - PMC - PubMed
    1. Bauernfeind A, Stemplinger I, Jungwirth R, Wilhelm R, Chong Y. Comparative characterization of the cephamycinase blaCMY-1 gene and its relationship with other β-lactamase genes. Antimicrob Agents Chemother. 1996;40:1926–1930. - PMC - PubMed
    1. Bauernfeind A, Chong Y, Schweighart S. Extended broad spectrum β-lactamase in Klebsiella pneumoniae including resistance to cephamycins. Infection. 1989;17:316–321. - PubMed
    1. Billot-Klein D, Gutmann L, Collatz E. Nucleotide sequence of the SHV-5 β-lactamase gene of a Klebsiella pneumoniae plasmid. Antimicrob Agents Chemother. 1990;34:2439–2441. - PMC - PubMed
    1. Coetzee J N, Datta N, Hedges R W. R factors from Proteus rettgeri. J Gen Microbiol. 1972;72:543–552. - PubMed

Publication types

LinkOut - more resources