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. 1998 May;36(5):1458-60.
doi: 10.1128/JCM.36.5.1458-1460.1998.

Rapid detection of species of the opportunistic yeast Trichosporon by PCR

T Sugita  1 , A Nishikawa, T Shinoda
Affiliations

Rapid detection of species of the opportunistic yeast Trichosporon by PCR

T Sugita et al. J Clin Microbiol. 1998 May.

Abstract

Trichosporon species are opportunistic pathogens, associated with a high mortality rate in immunocompromised patients. Oligonucleotide primers were used to amplify a 170-bp fragment of small-subunit ribosomal DNA of all species in the genus Trichosporon by PCR. The primers amplify DNAs of all species in the genus Trichosporon, including six causative agents of trichosporonosis. DNAs of other medically important yeasts, such as Candida albicans and Cryptococcus neoformans, are not amplified by this detection system.

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Figures

FIG. 1
FIG. 1
Agarose gel electrophoresis of PCR products of Trichosporon species and other medically important yeasts. Lane 1, molecular weight marker (HaeIII-digested φX174 DNA; Takara, Shiga, Japan); lane 2, T. cutaneum; lane 3, T. mucoides; lane 4, T. jirovecii; lane 5, T. moniliiforme; lane 6, T. asahii var. asahii; lane 7, T. asahii var. coremiformis; lane 8, T. asahii var. faecalis; lane 9, T. asteroides; lane 10, T. aquatile; lane 11, T. inkin; lane 12, T. ovoides; lane 13, T. brassicae; lane 14, T. domesticum; lane 15, T. montevideense; lane 16, T. dulcitum; lane 17, T. gracile; lane 18, T. loubieri var. loubieri; lane 19, T. loubieri var. laibachii; lane 20, T. sporotrichoides; lane 21, T. pullulans; lane 22, C. neoformans var. neoformans; lane 23, C. neoformans var. gattii; lane 24, Malassezia furfur; lane 25, Malassezia pachydermatis; lane 26, Candida albicans; lane 27, Candida glabrata; lane 28, Candida guilliermondii; lane 29, Candida kefyr; lane 30, Candida lusitaniae; lane 31, Candida parapsilosis; lane 32, Candida tropicalis; lane 33, Debaryomyces hansenii var. hansenii.
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