Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Apr;33(4):568-72.
doi: 10.1016/s0022-3468(98)90318-9.

TBJ murine neuroblastoma resists dibutyryl cyclic AMP-induced differentiation and effects on metalloproteinase secretion

C Fowler  1 , C Zimmer, S Zimmer
Affiliations

TBJ murine neuroblastoma resists dibutyryl cyclic AMP-induced differentiation and effects on metalloproteinase secretion

C Fowler et al. J Pediatr Surg. 1998 Apr.

Abstract

Background: Nonmetastatic C1300 murine neuroblastoma and TBJ, a metastatic variant, exhibit different mechanisms of metastasis and invasion.

Methods: In this study, they were examined for response to 1 mmol/L dibutyryl cyclic AMP in vitro.

Results: (1) dCAMP induced morphological differentiation in 20% to 40% of C1300 cells, whereas TBJ resisted differentiation. (2) Untreated TBJ expressed 92-kDa metalloproteinase, whereas C1300 expressed none. These results were not affected by dCAMP. (3) Growth curves of untreated C1300 and TBJ were exponential. Dibutyryl CAMP induced a plateau in C1300 growth that was confirmed by increased S-phase cells by cell cycle analysis.

Conclusions: TBJ growth was unaffected by dCAMP. Apoptosis, as assayed by Hoechst-merocyanine staining test, was not involved in the growth plateau.

PubMed Disclaimer

Publication types

LinkOut - more resources