[Study of SV40-transformed cell lines from rat dorsolateral prostate--analysis of growth factors and their receptors in the epithelial cell line]

Abstract

Background: We transformed primary cultured epithelial cells of the rat dorsolateral prostate by Simian Virus 40 (SV 40) and established a stable epithelial cell line (PESVH). To examine whether this cell line is available for the fundamental research of prostatic diseases, investigation on the growth factors and their receptors of this cell line was made.

Method: The [3H]-thymidine uptake method was used to examine the influence for cell proliferation of epidermal growth factor (EGF), acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF), keratinocyte growth factor (KGF), transforming growth factor (TGF)-alpha, TGF-beta 1 and androgens. The expression of mRNA for growth factors and their receptors was determined with Northern blot analysis.

Result: DNA synthesis was increased to 2.1-fold of control by the addition of 100 ng/ml EFG. The addition of aFGF (100 ng/ml), bFGF (100 ng/ml) and TGF-alpha (100 ng/ml) tended to stimulate the DNA synthesis, but not significantly. The addition of KGF (0.1-100 ng/ml) did not influence on the DNA synthesis of PESVH. The addition of testosterone and 5 alpha-dihydrotestosterone (10(-10)-10(-6) M) did not influence on the DNA synthesis. Northern blot analysis showed expression of mRNA for TGF-alpha, TGF-beta 1, EGF receptor and TGF-beta receptor (type II) in PESVH cell. These expressions were not influenced by the addition of androgens.

Conclusion: These findings suggested that PESVH cell could be used for one model of the androgen-independent prostatic cell.