doi: 10.1007/BF00356941.
Further studies on collagen mRNA: partial chemical characterization and polyadenylic acid sequence
- PMID: 958218
- DOI: 10.1007/BF00356941
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Further studies on collagen mRNA: partial chemical characterization and polyadenylic acid sequence
Mol Biol Rep.
1976 Jul.
Abstract
Collagen mRNA has already been purified and characterized by us. Its purity has now been enhanced by two different methods. Gel electrophoresis shows in either method, a single peak with the same mobility already reported: 1.05 X 10(6) daltons. Base composition analyses of collagen mRNA purified by either method were almost identical. Chemical analyses of the isolated polyadenylic acid stretch show that it is, 0.48 X 10(5) daltons-long, (about 140 nucleotides-long), contains 75% AMP, and is located at the 3' end of the polymer.
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