Oligonucleotide synthesis by the use of a 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBZ) group for the 5'-hydroxyl group of ribonucleoside and 2'-deoxyribonucleoside 3'-phosphoramidites
- PMID: 9585991
Oligonucleotide synthesis by the use of a 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBZ) group for the 5'-hydroxyl group of ribonucleoside and 2'-deoxyribonucleoside 3'-phosphoramidites
Abstract
Synthetic studies on the CpCpA terminus triplet, whose exocyclic amino groups are all labeled with 15N, and a phosphorodithioate-linked oligonucleotide in terms of 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) group as the novel base-labile protecting group for the 5'-hydroxyl groups of nucleoside 3'-phosphoramidites, are described.