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. 1998 May;42(5):1259-62.
doi: 10.1128/AAC.42.5.1259.

Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A beta-lactamase (CTX-M-4): involvement of serine 237 in cephalosporin hydrolysis

M Gazouli  1 , E Tzelepi, S V Sidorenko, L S Tzouvelekis
Affiliations

Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A beta-lactamase (CTX-M-4): involvement of serine 237 in cephalosporin hydrolysis

M Gazouli et al. Antimicrob Agents Chemother. 1998 May.

Abstract

The sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-4) was determined. It was located in a plasmid harbored by a Salmonella typhimurium strain. CTX-M-4 was similar to the plasmidic cefotaxime-hydrolyzing beta-lactamases CTX-M-2 and Toho-1 and related to the chromosomal beta-lactamase of Klebsiella oxytoca. A Ser-237-->Ala substitution, introduced by site-directed mutagenesis, caused minor alterations in the interaction of CTX-M-4 with beta-lactams, reducing slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate.

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Figures

FIG. 1
FIG. 1
Nucleotide and deduced amino acid sequences of CTX-M-4 β-lactamase. The conserved residues of the class A β-lactamases are underlined. The signal peptide is indicated by an exclamation mark. The Ambler numbering scheme was followed.
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References

    1. Ambler R P, Coulson A F W, Frère J-M, Ghuysen J-M, Joris B, Forsman M, Levesque R C, Tiraby G, Waley S G. A standard numbering scheme for the class A β-lactamases. Biochem J. 1991;276:269–270. - PMC - PubMed
    1. Arakawa Y, Ohta M, Kido N, Mori M, Ito H, Komatsu T, Fujii Y, Kato N. Chromosomal β-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum β-lactam antibiotics. Antimicrob Agents Chemother. 1989;33:63–70. - PMC - PubMed
    1. Barthélémy M, Péduzzi J, Bernard H, Tancrède C, Labia R. Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum β-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca. Biochim Biophys Acta. 1992;1122:15–22. - PubMed
    1. Bauernfeind A, Caselas J M, Goldberg M, Holley M, Jungwirth R, Mangold P, Röhnisch T, Schweighart S, Wilhelm R. A new plasmidic cefotaximase from patients infected with Salmonella typhimurium. Infection. 1992;20:158–163. - PubMed
    1. Bauernfeind A, Grimm H, Schweighart S. A new plasmidic cefotaximase in a clinical isolate of Escherichia coli. Infection. 1990;18:294–298. - PubMed

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