[Establishment and characterization of the SV40-transformed stromal cell line from rat dorsolateral prostate]

Abstract

Background: The SV40-transformed stromal cell line (PSSVH) was established from rat dorsolateral prostate. To clarify the usefulness, PSSVH was studied on the immunohistochemical characteristics and the mRNA expression of growth factors and their receptors.

Method: Immunohistochemical method was used to detect cytokeratin, vimentin and large T antigen in the PSSVH cells. Chromosomal analyses were carried out by the Giemsa-banding technique. [3H]-thymidine uptake method was used to examine the influence for cell proliferation of androgens. To examine the expression of mRNA for epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), keratinocyte growth factor (KGF), transforming growth factor (TGF)-alpha, TGF-beta 1 and their receptors, poly (A) RNA was extracted from PSSVH cells and Northern blot analysis was done.

Result: Immunohistochemically, PSSVH was positive for large T antigen, and the cell had a character of stromal cell which included a negative result for cytokeratin and a positive for vimentin. The cell had a karyotype of aneuploid, whereas it had not a common marker chromosome. The addition of testosterone (T: 10(-10)-10(-6) M) and 5 alpha-dihydrotestosterone (DHT: 10(-10)-10(-6) M) did not influence on the DNA synthesis of PSSVH cells. Northern blot analysis showed expression of mRNA for KGF, TGF-alpha, TGF-beta 1, EGF receptor and TGF-beta receptor (type II) in PSSVH cells. These expressions were not influenced by the addition of androgens.

Conclusion: PSSVH is the androgen-independent stromal cell from rat dorsolateral prostate and may be useful for the fundamental study of the prostate.