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. 1998 Apr;35(4):265-8.
doi: 10.1136/jmg.35.4.265.

A simple and efficient method for microdissection and microFISH

J J Engelen  1 , J C Albrechts, G J Hamers, J P Geraedts
Affiliations

A simple and efficient method for microdissection and microFISH

J J Engelen et al. J Med Genet. 1998 Apr.

Abstract

A simple and efficient method for the dissection of (marker) chromosomes, (micro)nuclei, and chromosome regions is presented. Before microdissection, metaphases are overlaid with milli-Q water to rehydrate the chromosomes, which makes them soft and sticky. The dissected chromosome fragments are dissolved without proteinase-K or topoisomerase treatment and directly amplified using a degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR). The advantages of this microFISH method over previously reported methods are: (1) microdissection in this way is very fast; (2) a chromosome, marker, (micro)nucleus, or chromosome region is collected as a whole using only one microneedle; (3) the dissected material sticks tightly to the needle without the risk of getting lost; (4) no Sequenase is used in the DOP-PCR reaction which reduces the risk of contamination.

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References

    1. Hum Genet. 1992 Apr;89(1):13-7 - PubMed
    1. Hum Genet. 1990 May;84(6):507-11 - PubMed
    1. J Biochem. 1992 Jul;112(1):75-80 - PubMed
    1. Nat Genet. 1992 Apr;1(1):24-8 - PubMed
    1. Hum Mol Genet. 1993 Aug;2(8):1117-21 - PubMed

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