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. 1978 Apr;3(2):123-34.
doi: 10.1016/0378-1119(78)90056-2.

Cloned seventeen-nucleotide-long synthetic lactose operator is biologically active

C P Bahl, R Wu, S Narang

Cloned seventeen-nucleotide-long synthetic lactose operator is biologically active

C P Bahl et al. Gene. 1978 Apr.

Abstract

A 17-nucleotide-long synthetic DNA molecule constituting the minimal recognition sequence of the lactose operator has been cloned in E. coli using the vehicle pBR313 and a synthetic HindIII adaptor. The clones containing the lac-pBR313 hybrid DNA constitutively produced beta-galactosidase. The level of beta-galactosidase was high and comparable to that obtained in cells carrying a 21-nucleotide-long synthetic lac operator on pMB9 plasmid or cells carrying a natural lac operator on pOP203-1 plasmid.

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