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. 1998 Jun;64(6):2278-80.
doi: 10.1128/AEM.64.6.2278-2280.1998.

Detection of Toxoplasma gondii oocysts in drinking water

J Isaac-Renton  1 , W R Bowie, A King, G S Irwin, C S Ong, C P Fung, M O Shokeir, J P Dubey
Affiliations

Detection of Toxoplasma gondii oocysts in drinking water

J Isaac-Renton et al. Appl Environ Microbiol. 1998 Jun.

Abstract

The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gondii. The method developed was based on the current U.S. Environmental Protection Agency method for detection of Cryptosporidium oocysts. Collection of large-volume drinking water samples and cartridge filter processing were unchanged, although identification of Toxoplasma oocysts in the filter retentate was carried out by using a previously described rodent model. Validation of the method developed was tested by using oocysts from a well-characterized Toxoplasma strain.

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References

    1. American Society for Testing and Materials. Annual book of ASTM standards, section 1, water and environmental technology. Vol. 11.02. Washington, D.C: American Society for Testing and Materials; 1992. D-19 proposal P229. Proposed test method for Giardia cysts and Cryptosporidium oocysts in low turbidity water by a fluorescent antibody method; pp. 925–935.
    1. Beneson M W, Takafuji E T, Lemon S M, Greenup R L, Sultzer A J. Oocyst-transmitted toxoplasmosis associated with ingestion of contaminated water. N Engl J Med. 1982;300:694–699. - PubMed
    1. Bowie W R, King A S, Werker D H, Isaac-Renton J, Bell A A, Eng S B, Marion S. Outbreak of toxoplasmosis associated with municipal drinking water. Lancet. 1997;350:173–177. - PubMed
    1. Dubey J P, Desmonts G. Serological responses of equids fed Toxoplasma gondii oocysts. Equine Vet J. 1987;19:337–339. - PubMed
    1. Dubey J P, Speer C A, Shen S K, Kwok O C H, Blixt J A. Oocyst-induced murine toxoplasmosis: life cycle, pathogenicity, and stage conversion in mice fed Toxoplasma gondii oocysts. J Parasitol. 1997;83:870–882. - PubMed

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