Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 1998 May;15(5):302-7.
doi: 10.1023/a:1022548612107.

Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases

M Boada  1 , M Carrera, C De La Iglesia, M Sandalinas, P N Barri, A Veiga
Affiliations
Case Reports

Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases

M Boada et al. J Assist Reprod Genet. 1998 May.

Abstract

Purpose: The use of Tyrode's acid to drill the zona pellucida for embryo biopsy is the most widely used methodology in preimplantation genetic diagnosis. Instead of this, we propose the use of a 1.48-micron diode noncontact laser, which is quicker, simpler, and safer.

Methods: The laser beam was tangentially guided to the zona pellucida of the embryo. Depending on zona pellucida measurement, two to four consecutive shots of 8-22 msec were necessary to drill the zona pellucida of the 13 embryos biopsied for two patients (hemophilia carriers).

Results: Female embryos were replaced into the uterus of the patients (1.5 embryos/replacement). One single pregnancy was established (33.3% implantation rate). Coculture of untransferable embryos showed a blastocyst rate of 66.7% (4/6) for male embryos and 25% (1/4) for abnormal ones.

Conclusions: These results demonstrate the safety and usefulness of laser methodology in preimplantation genetic diagnosis.

PubMed Disclaimer

References

    1. Edwards RG. Maturation in vitro of human ovarian oocytes. Lancet. 1965;2:926–929. - PubMed
    1. Handyside AH, Kontogianni FH, Hardy K, Winston RML. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990;244:768–770. - PubMed
    1. Liu J, Lissens W, Devroey P, Van Steirteghem A, Liebaers I. Efficiency and accuracy of polymerase-chain-reation assay for cystic fibrosis allele delta F508 in single cell. Lancet. 1992;339:1190–1192. - PubMed
    1. Handyside AH, Lesko JG, Tarin JJ, Winston RML, Hughes MR. Birth of a normal girl after in vitro fertilisation and preimplantation diagnostic testing for cystic fibrosis. N Engl J Med. 1992;327:905–909. - PubMed
    1. Munné S, Lee A, Rosenwaks Z, Grifó J, Cohen J. Diagnosis of major chromosome aneuploidies in human preimplantation embryos. Hum Reprod. 1993;8:2185–2191. - PubMed

Publication types