Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1998 May;5(3):412-4.
doi: 10.1128/CDLI.5.3.412-414.1998.

Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods

F Guscetti  1 , C Bernasconi, K Tobler, K Van Reeth, A Pospischil, M Ackermann
Affiliations
Comparative Study

Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods

F Guscetti et al. Clin Diagn Lab Immunol. 1998 May.

Abstract

An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Performance of different tests for detection of PEDV.
See this image and copyright information in PMC

References

    1. Bernasconi C. Dissertation thesis. Zurich, Switzerland: University of Zurich; 1996.
    1. Bernasconi C, Guscetti F, Utiger A, Van Reeth K, Ackermann M, Pospischil A. Experimental infection of gnotobiotic piglets with a cell culture adapted porcine epidemic diarrhoea virus: clinical, histopathological, and immunohistochemical findings. In: Schwyzer M, Ackermann M, Bertoni G, Kocherhans R, McCullogh K, Engels M, Wittek R, Zanoni R, editors. Immunobiology of viral infections. Proceedings of the Third Congress of the European Society for Veterinary Virology. 1995. pp. 542–546.
    1. Callebaut P, Debouck P, Pensaert M. Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea. Vet Microbiol. 1982;7:295–306. - PMC - PubMed
    1. Carvajal A, Lanza I, Diego R, Rubio P, Carmenes P. Evaluation of a blocking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhea virus and its antibodies. J Vet Diagn Invest. 1995;7:60–64. - PubMed
    1. Coussement W, Ducatelle R, Debouck P, Hoorens J. Pathology of experimental CV777 coronavirus enteritis in piglets. I. Histological and histochemical study. Vet Pathol. 1982;19:46–56. - PubMed

Publication types