Disc agar diffusion antimicrobial susceptibility tests with beta-lactamase producing Neisseria gonorrhoeae

Abstract

The emergence of beta-lactamase-producing strains of Neisseria gonorrhoeae has led to a reexamination of the role of the disc agar diffusion method in susceptibility testing of gonococci. Our data show that the disc agar diffusion test can be used to screen for beta-lactamase production by these organisms. The disc tests were done on GC Agar Base supplemented with 1% IsoVitaleX. An inoculum of 10(8) colony forming units/ml and either a 10-unit-penicillin or a 10-microgram-ampicillin disc were used. A zone diameter of less than or equal to 19 mm was indicative of beta-lactamase production. These results were compared with results of chemical tests for beta-lactamase and with minimal inhibitory concentrations. Recommondations were also made for a disc test with tetracycline and spectinomycin, but these methods must remain tentative because of the lack of resistant strains.