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. 1978 Jun;134(3):875-83.
doi: 10.1128/jb.134.3.875-883.1978.

Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny

R V Miller, C M Ku

Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny

R V Miller et al. J Bacteriol. 1978 Jun.

Abstract

Mutants of Pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (Les-) have been isolated. These les mutations map to two areas of the P. aeruginosa chromosomal map as determined by conjugational and transductional analyses. Two phenotypic classes of Les- mutants were identified. One class of mutations has pleiotropic effects on DNA metabolism. These mutants are unable to recombine genetic material acquired as a result of either conjugation or transduction (Rec-). In addition, the ability of these Les- Rec- mutants to repair UV-induced damage to bacteriophage is reduced (host-cell reactivation deficient, Hcr-). Mutants of the second class are Les-, Rec+, and Hcr+.

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