Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy
- PMID: 961433
- DOI: 10.1111/j.1699-0463.1976.tb01929.x
Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy
Abstract
In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.
Similar articles
-
Family infections by reo-like virus. Comparison of antibody titres by complement fixation and immunoelectroosmophoresis.Scand J Infect Dis. 1977;9(4):257-61. doi: 10.3109/inf.1977.9.issue-4.01. Scand J Infect Dis. 1977. PMID: 203024
-
Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis.J Gen Virol. 1977 May;35(2):203-18. doi: 10.1099/0022-1317-35-2-203. J Gen Virol. 1977. PMID: 406356
-
Occurrence of reo-like calf viruses in young children with acute gastroenteritis. Diagnoses established by electron microscopy and complement fixation, using the reo-like virus as antigen.Acta Pathol Microbiol Scand B. 1976 Feb;84(1):22-8. Acta Pathol Microbiol Scand B. 1976. PMID: 175639
-
Comparison of solid-phase immune electron microscopy, direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in faecal samples.J Virol Methods. 1982 Feb;4(1):45-53. doi: 10.1016/0166-0934(82)90053-2. J Virol Methods. 1982. PMID: 6277978
-
Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis.Lancet. 1974 Feb 2;1(7849):149-51. doi: 10.1016/s0140-6736(74)92440-4. Lancet. 1974. PMID: 4129719 No abstract available.
Cited by
-
Human rotavirus antigen detection by enzyme-immunoassay with antisera against Nebraska calf diarrhoea virus.J Clin Pathol. 1981 Jun;34(6):680-5. doi: 10.1136/jcp.34.6.680. J Clin Pathol. 1981. PMID: 6265506 Free PMC article.
-
Seasonal fluctuations in the occurrence of enterotoxigenic bacteria and rotavirus in paediatric diarrhoea in Addis Ababa.Bull World Health Organ. 1981;59(1):67-73. Bull World Health Organ. 1981. PMID: 6266682 Free PMC article.
-
Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves.J Clin Microbiol. 1977 Nov;6(5):530-2. doi: 10.1128/jcm.6.5.530-532.1977. J Clin Microbiol. 1977. PMID: 200635 Free PMC article.
-
Diagnosis of human rotavirus infections: comparison of an electrophoretic method, a modified complement fixation test and electron microscopy for rotavirus detection.Arch Virol. 1981;67(4):341-4. doi: 10.1007/BF01314837. Arch Virol. 1981. PMID: 6263229
-
The rotaviruses.Arch Virol. 1978;57(1):1-23. doi: 10.1007/BF01315633. Arch Virol. 1978. PMID: 77663 Free PMC article. No abstract available.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources