EMF genes interact with late-flowering genes to regulate Arabidopsis shoot development

Abstract

To investigate the genetic mechanisms regulating the transition from vegetative to reproductive phase in Arabidopsis, double mutants between two embryonic flower (emf) and 12 different late-flowering mutants were constructed and analyzed. Double mutants in all combinations displayed the emf phenotypes without forming rosettes during early development; however, clear variations between different double mutants were observed during late development. fwa significantly enhanced the vegetative property of both emf mutants by producing a high number of sessile leaves without any further reproductive growth in emf1 fwa double mutants. It also produced numerous leaf-like flower structures similar to those in leafy ap1 double mutant in emf2 fwa double mutants. Nine late-flowering mutants, ft, fca, ld, fd, fpa, fe, fy, fha, and fve, caused different degrees of increase in the number of sessile leaves, the size of inflorescence, and the number of flowers only in weak emf1 and emf2 mutant alleles background. Two late-flowering mutants, co and gi, however, had no effect on either emf1 and emf2 mutant alleles in double mutants. Our results suggest that FWA function in distinct pathways from both EMF genes to regulate flower competence by activating genes which specify floral meristem identity. CO and GI negatively regulate both EMF genes, whereas the other nine late-flowering genes may interact with EMF genes directly or indirectly to regulate shoot maturation in Arabidopsis.