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. 1978 May;26(5):412-4.
doi: 10.1177/26.5.96177.

A new method for the study of glutaraldehyde-induced crosslinking properties in proteins with special reference to the reaction with amino groups

S O Molin, H Nygren, L Dolonius

A new method for the study of glutaraldehyde-induced crosslinking properties in proteins with special reference to the reaction with amino groups

S O Molin et al. J Histochem Cytochem. 1978 May.

Abstract

A new method for the study of glutaraldehyde reactions with proteins is presented. Glutaraldehyde-reacted protein is in a first step isolated and then in a second step reacted with aminohexyl groups bound to Sepharose particles. This reaction is linear at low protein concentrations and proceeds rapidly when proteins are reacted with 100-fold and 1000-fold molar excess of glutaraldehyde. This method enables the study of glutaraldehyde-induced crosslinking properties of the modified proteins as an isolated property with high reliability.

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