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. 1976 Aug;3(8):2089-99.
doi: 10.1093/nar/3.8.2089.

Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid

M Ikehara, T Fukui, N Kakiuchi
Free PMC article

Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid

M Ikehara et al. Nucleic Acids Res. 1976 Aug.
Free PMC article

Abstract

Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ellipticity. Titration of poly (Az) with HCl gave a transition at pH 5.5, but exact structure of the acid-form complex was not elucidated. Upon mixing with poly (U), poly (Az) forms a 1:1 and 1:2 complexes having Tm's somewhat higher than that of poly (A)- poly (U) complex in the same condition.

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