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Review
. 1998 Jun;36(6):1471-9.
doi: 10.1128/JCM.36.6.1471-1479.1998.

Determinations of levels of human immunodeficiency virus type 1 RNA in plasma: reassessment of parameters affecting assay outcome. TUBE Meeting Workshop Attendees. Technology Utilization for HIV-1 Blood Evaluation and Standardization in Pediatrics

J Lew  1 , P Reichelderfer, M Fowler, J Bremer, R Carrol, S Cassol, D Chernoff, R Coombs, M Cronin, R Dickover, S Fiscus, S Herman, B Jackson, J Kornegay, A Kovacs, K McIntosh, W Meyer, N Michael, L Mofenson, J Moye, T Quinn, M Robb, M Vahey, B Weiser, T Yeghiazarian
Affiliations
Review

Determinations of levels of human immunodeficiency virus type 1 RNA in plasma: reassessment of parameters affecting assay outcome. TUBE Meeting Workshop Attendees. Technology Utilization for HIV-1 Blood Evaluation and Standardization in Pediatrics

J Lew et al. J Clin Microbiol. 1998 Jun.
No abstract available

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Figures

FIG. 1
FIG. 1
Intraassay variability in plasma HIV-1 RNA levels for five replicates of three samples. The Roche Amplicor Monitor assay was used to quantitate plasma HIV-1 RNA levels (copies per milliliter) by two different experienced technicians in separate sample runs. The mean number of RNA copies per milliliter and the standard deviation (SD) (log10) for each sample run, by technician, are located within the columns and above the columns, respectively.
FIG. 2
FIG. 2
Comparison of two RNA extraction methods, heparinase treatment and silica-binding extraction, on HIV-1 RNA copy number detected by the Roche Amplicor Monitor assay.
FIG. 3
FIG. 3
Geometric mean baseline HIV-1 RNA level by year of study entry. The collected blood specimens were separated, and the serum was initially stored at −20 or −70°C prior to periodic shipment to a central repository. Serum specimens were stored centrally at −70°C for 4 to 7 years prior to batch testing by the Organon Teknika NASBA assay (28).
FIG. 4
FIG. 4
Percent difference in HIV-1 RNA detection between one and three freeze-thaw cycles. The RNA equivalents per milliliter after the first freeze-thaw cycle, which is considered the baseline value, are given beneath the white columns (freezing at −20°C) and beneath the black columns (freezing at −80°C; numbers in brackets). HIV-1 RNA was detected by the Chiron bDNA assay (37).
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